Fig. 2.

Subscreening of the Medaka cDNA library. a From the cDNA pools that were considered to contain regulators of p53 and/or Mdm2, the individual bacteria were amplified from the master library. The plasmids were purified and 12.5 ng of the individual cDNAs were transfected together with p53 (5 ng), mdm2 (45 ng) and myc-ror2 (5 ng) into H1299 cells in a 96-well format. 24 h after transfection, cells were analyzed by Western Blotting. b H1299 cells were transfected with plasmids encoding p53, Mdm2 and Myc-ROR2 together with the individual clones of the indicated pool hit or with plasmids encoding p53, Mdm2 and Myc-ROR2 without cDNA clones, for control (ctrl). 24 h after transfection, cells were harvested and analyzed as described in the legend to Fig. 1