Fig. 5.

Effects of cofilin-1 knockdown on Angiotensin II-induced RelA/p65 nuclear translocation and NF-κB activity. After transfection and passage, cells were challenged for 12 h with AngII (10⁻⁶mol L⁻¹). a–d Cytoplasmic and nuclear extracts were prepared and assayed for p65 and Iκ-Bα protein levels. The bar graph represents the effect of cofilin-1 depletion on AngII-induced p65 nuclear distribution and Iκ-Bα degradation. e Luciferase dual reporter gene assay of NF-κB activity. Cells in group a, c were transfected Lv-control-shRNA and Lv-cofilin1-shRNA respectively, then stimulated by AngII. Cells in b and d groups were stimulated by AngII without transfection. Cells in group e were preincubated with 50 μg mL⁻¹ GSPE, then stimulated by AngII, while group f was normal control. The data are mean ± SD. *P < 0.05, **P < 0.01, vs. group f. ^#P < 0.05, ^##P < 0.01, vs. group c