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. 2015 Oct 9;5:57. doi: 10.1186/s13578-015-0048-4

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© Chollet et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — PBA treatment caused some localization of PC_wt in GRASP55 positive vesicles. Confocal images from PC (green) (a) and GRASP55 (red) (b) stained CHO-K1 cells stably transfected with PC_wt and treated with 5 mM PBA for 48 h. Cells were stained with rabbit polyclonal anti-PC (a), and mouse monoclonal anti-GRASP55 (b). Merged images of a and b are shown in c, with co-localized green and red pixels shown in yellow color. Three independent experiments were performed. Bar in a–c 5 µm