Skip to main content
. Author manuscript; available in PMC: 2015 Nov 1.
Published in final edited form as: Plant Biotechnol J. 2014 Nov 18;13(4):540–550. doi: 10.1111/pbi.12278

Figure 2.

Figure 2

Construction of pAlgaeDGAT-eGFP vector to transform the Chlamydomonas reinhardtii cells. The synthetic cassette containing BnDGAT2-6XHIS-Tag-KDEL-NOS-PolyA-35Sde-eGFP was cloned in pChlamy_1 at restriction sites XbaI and NotI. BnDGAT2 (Accession No. AF155224) was expressed by Hsp70-RbcS2 promoter and was tagged with 6XHis and KDEL sequences at the C-terminal. Enhanced GFP (Accession No. JN596101) was expressed by a double enhancer 35Sde promoter (Accession No. V00140.1). The letters F and R above DGAT2 gene denote the forward and reverse PCR primers used in the preliminary assessment of transgene integration in algal cells.