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. 2015 Oct 9;10(10):e0140321. doi: 10.1371/journal.pone.0140321

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© 2015 Gil et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 5 — (A) Aliquots from a DRM fraction (#3) and a soluble fraction (#12, SF) from synaptosomal preparations (see Fig 1) were treated with 10 U/mL ARSA, lipids were subsequently extracted and spotted onto a Hybond-c membrane. Binding of proEtx to the processed lipids was then assessed with PLO assay. A representative result is shown. (B) Quantification of the results shown in (A). The signal intensity from untreated DRM samples is taken as 100%. Mean ± SD from three experiments is shown. (C) Binding of proEtx to increasing amounts of galactosylceramide (GC), together with 100 pmols of sulfatide as positive control, was analyzed with the PLO assay. The results show the absence of binding of Etx to GC.