Table 1. Estimate of L. mexicana RNA in mixed RNA samples from infected BMDMs.

Sample	AMA1	AMA2	AMA3
Amastigotes per 100 macrophages	207	636	748
LSU β (1.6 kb) peak size in AFU	3.4	4.8	8.4
28S (4.7 kb) peak size in AFU	36.6	16.5	17.5
Relative L. mexicana rRNA amount	2.1	3.0	5.3
Relative murine rRNA amount	7.8	3.5	3.7
Estimated proportion of L. mexicana RNA in sample	21.2%	46.1%	58.9%
Percentage of reads mapping to L. mexicana genome	25.6%	54.9%	61.2%

Peak sizes of the LSU β and 28S rRNA subunits were measured in arbitrary fluorescence units (AFU) on an Agilent Bioanalyzer. To estimate the proportion of L. mexicana RNA in the sample, AFU were divided by length of the corresponding rRNA to determine the relative amounts of leishmanial and murine rRNAs. The number of amastigotes per 100 macrophages was determined by light microscopy.