Table 2. RNA-seq read mapping summary.
Sample | PRO1 | PRO2 | PRO3 | AXA1 | AXA2 | AXA3 | AMA1 | AMA2 | AMA3 |
---|---|---|---|---|---|---|---|---|---|
Random primed library | |||||||||
Total paired-end reads | 12,835,963 | 13,087,624 | 12,858,646 | 13,124,114 | 13,748,422 | 12,778,984 | 13,477,640 | 13,357,246 | 13,044,810 |
Low-quality reads | 20,538 | 23,558 | 29,575 | 17,061 | 34,371 | 17,891 | 9,434 | 13,357 | 11,740 |
Reads mapping to multiple loci | 4,133,727 | 3,572,451 | 4,109,315 | 5,155,858 | 4,552,037 | 4,044,110 | 1,187,608 | 2,910,706 | 3,115,683 |
Genes with non zero read counts | 9,129 | 9,119 | 9,119 | 9,133 | 9,129 | 9,125 | 9,112 | 9,123 | 9,123 |
Total reads mapped to L. mexicana genome | 12,220,790 | 12,301,132 | 12,109,395 | 12,525,089 | 12,950,160 | 11,893,323 | 3,437,982 | 7,224,190 | 7,767,651 |
Reads with no match in genome | 594,635 | 762,934 | 719,676 | 581,964 | 763,891 | 867,770 | 10,030,224 | 6,119,699 | 5,265,419 |
Total reads with SL sequence (last 12nt) | 673,297 | 1,067,867 | 937,353 | 786,722 | 1,009,393 | 1,225,884 | 262,651 | 499,028 | 572,112 |
Mapped reads with SL sequence (last 12nt) | 665,046 | 1,051,560 | 923,254 | 778,432 | 994,815 | 1,208,732 | 259,961 | 493,948 | 566,435 |
T15VN PRIMED LIBRARY | |||||||||
Total reads | nd | 25,956,366 | 22,854,437 | nd | 21,935,789 | 20,450,384 | nd | 23,956,594 | 25,696,946 |
Low-quality reads | nd | 8,012,973 | 8,617,839 | nd | 6,910,070 | 5,436,250 | nd | 9,613,243 | 8,996,465 |
Total reads with poly(A) tail (≥5 A) | nd | 2,097,561 | 2,088,542 | nd | 2,200,926 | 2,149,944 | nd | 1,793,186 | 1,476,286 |
Mapped reads with poly(A) tail (≥5 A) | nd | 756,459 | 767,004 | nd | 805,680 | 812,005 | nd | 424,491 | 373,912 |