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. 2015 Oct 9;11(10):e1005174. doi: 10.1371/journal.ppat.1005174

Fig 5. Binding of TAX1BP1, NDP52 and optineurin to different ubiquitin chain types.

Fig 5

GFP immunoprecipitation followed by either linear tetra-ubiquitin (A), K48-linked polyubiquitin (B), or K63-linked polyubiquitin (C) pull-downs (PD) from RPE cells transfected with GFP alone, GFP-TAX1BP1, GFP-NDP52, or GFP-optineurin wild-type and mutants. Western blot analysis performed using antibodies specific for ubiquitin and GFP.