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. 2015 Oct 9;11(10):e1005174. doi: 10.1371/journal.ppat.1005174

Fig 7. TAX1BP1 interacts with LC3B as well as LC3C and requires its zinc finger domains to localise to ubiquitylated Salmonella.

Fig 7

(A) Mammalian 2-hybrid assay in CHOK.1 cells with LC3A, B, or C and GABARAP, GABARAPL1 or L2 as bait and TAX1BP1 full-length wild-type as prey. Results are represented as the normalised luciferase activity against a bait only control. (B) Mammalian 2-hybrid assay in CHOK.1 cells using LC3B or LC3C as bait and full-length TAX1BP1, NDP52, optineurin wild-type and LIR mutants as prey. Results are represented as the normalised luciferase activity against a bait only control. (C) Mammalian 2-hybrid assay in CHOK.1 cells with LC3B as bait and TAX1BP1 full-length wild-type and mutants as prey. Results are represented as the normalised luciferase activity against a bait only control. (D) HeLa cells transfected with GFP-TAX1BP1 wild-type or V144S LIR mutant, Q743A/E747K/Q770A/E774K double zinc finger mutant, and V144S/Q743A/E747K/Q770A/E774K LIR and double zinc finger mutant were infected with mCherry expressing Salmonella for 1 hour prior to saponin extraction and fixation. Cells were immunostained for GFP and ubiquitin and processed for confocal microscopy. Nuclei are labelled with Hoechst (blue). Scale bar, 20 μm.