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. Author manuscript; available in PMC: 2016 Apr 1.
Published in final edited form as: Gene Ther. 2015 May 25;22(10):811–821. doi: 10.1038/gt.2015.51

Figure 6. Regenerate axons following PCC treatment form synapses in the SCN.

Figure 6

(a) Schematic coronal brain section showing the SCN marked in a dotted red rectangle. (b) CTB-labeled axons (red) in the SCN express presynaptic marker Vglut2 (green). (b′) High-magnifications of the boxed area in (b), with XZ and YZ projections at the yellow crosshairs. (c) Retinal cross section showing RGCs expressing WGA (green) six weeks following AAV-Cre injection in iZ/WAP mice. DAPI (blue) is used in these tissue sections for nuclear staining. (d) WGA+ cells in the SCN of an uninjured animal. WGA (green) and DAPI (blue). (e) WGA+ cells in the LGN (as marked by dotted line) of an uninjured animal. WGA (green) and DAPI (blue). (f) No WGA+ cells are detected in the injured AAV-EGFP control mice (n=5). (g) Low magnification coronal brain section showing the SCN of an injured PCC animal, marked in a yellow circles immunostained with antibodies against WGA. CTB, red; DAPI, blue. (h) High magnification of the dotted area in (g) showing CTB+ axons (red) wrapping or making close contact with WGA+ cells in the SCN. Scale bars, 50 μm.