Skip to main content
. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Hypertension. 2015 Sep 8;66(5):1014–1022. doi: 10.1161/HYPERTENSIONAHA.115.05993

Figure 2. Genotyping of secondary nodules identified in adrenals with APA. A. Adrenal 1.

Figure 2

Left and middle panels: Sanger sequencing chromatograms showing the KCNJ5 mutation c.451G>A in APA and the wild type KCNJ5 sequence in the secondary nodule. Right panel: ddPCR analysis showing the amount of wild type G allele and mutant A allele of the KCNJ5 mutation c.451G>A in the secondary nodule. B. Adrenal 26. Left panels: Sanger sequencing chromatograms showing the KCNJ5 mutation c.451G>C in APA and the wild type KCNJ5 sequence in the secondary nodule. Right panels: ddPCR analysis showing the amount of wild type G allele and mutant A allele (c.451G>A) and the amount of wild type G allele and mutant C allele (c.451G>C) in the secondary nodule. C. Adrenal 15. Left and middle panels: Sanger sequencing chromatograms showing the wild type KCNJ5 sequence in APA, the CACNA1D mutation c.2293C>T in APA, and the KCNJ5 mutation c.451G>A in the secondary nodule. Right panel: ddPCR analysis showing the amount of wild type G allele and mutant A allele of the KCNJ5 mutation c.451G>A in the secondary nodule. D. Adrenal 24. Left and middle panels: Sanger sequencing chromatograms showing the wild type KCNJ5 sequence in APA, the CACNA1D mutation c.2293C>T in APA, and the KCNJ5 mutation c.1207G>C in the secondary nodule. Right panel: ddPCR analysis showing the amount of wild type G allele and mutant A allele of the KCNJ5 mutation c.451G>A in the secondary nodule. E. Adrenal 4. Left and middle panels: Sanger sequencing chromatograms showing the wild type KCNJ5 sequence in APA and the KCNJ5 mutation c.451G>A in the secondary nodule. Right panel: ddPCR analysis showing the amount of wild type G allele and mutant A allele of the KCNJ5 mutation c.451G>A in the secondary nodule.