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. Author manuscript; available in PMC: 2015 Dec 5.
Published in final edited form as: Environ Microbiol. 2015 May 8;17(11):4415–4428. doi: 10.1111/1462-2920.12874

Figure 5. Sensitivity tests of the wild-type, cpxA null mutant, cpxR null mutant and complemented strains to H2O2 or polymyxin B.

Figure 5

(A) For sensitivity to H2O2, bacteria were grown until stationary-phase in M63 glycerol medium. The culture was incubated with 3 mM of H2O2 and survivals were determined by numeration. Survival rate was expressed as the number of CFU counted at each time/the number of CFU before addition of H2O2. Results are the average of three independent experiments (B) For sensitivity to polymyxin B, bacteria were grown until stationary phase in M63 glycerol medium. The culture was incubated with the indicated concentrations and survivals were determined by numeration. Survival rate was expressed as the number of CFU counted at each time/the number of CFU before addition of polymyxin B. Results are the average of three independent experiments. Asterisks indicate that a significant difference exists only between the wild-type and the cpxA or cpxR null mutants.