TABLE 1 .
Plasmids and strains used in this study
| Strain or plasmid | Description or genotype | Purpose or description | Reference or source |
|---|---|---|---|
| Plasmids | |||
| pMTFCHA | Pfdx Mevr | Expression vector | 52 |
| pRSK01 | pNBK07::attB1,attB2, Mevr Ura+ | Genomic integration vector | 52 |
| pKAD01 | pRSK01::ΔhpyA | Deletion of hpyA | This study |
| pKAD02 | pMTFCHA::hpyA | Overexpression of hpyA | This study |
| pKAD03 | pMTFCHA::Prpa200::hpyA | Complementation of ΔhpyA | This study |
| Strains | |||
| MDK407 | Δura3 | Parent strain, Ura− | 53 |
| KAD100 | Δura3 ΔhpyA | Histone null mutant, Ura− | This study |
| KAD101 | Δura3/pMTFCHA | Ura− Mevr | This study |
| KAD102 | Δura3/pKAD02 | hpyA overexpression, Ura− Mevr | This study |
| KAD103 | Δura3 ΔhpyA/pKAD03 | ΔhpyA complementation, Ura− Mevr | This study |