FIG 1 .

Complementation of yeast pex22 mutant by dual expression of FAM1 and CoPEX4. Wild-type S. cerevisiae and Δpex22 mutants expressing GFP-PTS1, a fluorescent peroxisome marker, were transformed with vectors harboring FAM1 or CoPEX4 or empty vector. In the wild type, GFP localized at peroxisomes, producing a punctate pattern, while in Δpex22 mutants, GFP was uniformly distributed in the cytoplasm, indicating peroxisome deficiency. Introduction of FAM1 into the Δpex22 mutant partially restored localization of GFP-PTS1 to peroxisomes, while introduction of FAM1 together with CoPEX4 provided full restoration, producing the same punctate GFP fluorescence pattern as in the wild type. Bars, 5 µm. DIC, differential interference contrast.