FIG 9 .

Proposed model for the import of Woronin body matrix proteins and receptor recycling in C. orbiculare. Woronin body matrix protein Hex1 binds the Pex5 receptor in the cytosol via its C-terminal peroxisome targeting signal (PTS1). The Pex5-cargo complex first interacts with docking proteins Pex13 and Pex14 on the peroxisome surface and then translocates into the peroxisome matrix, mediated by Pex2, Pex8, Pex10, and Pex12. Dissociation of the Pex5 receptor from Hex1 occurs after sequestration of the complex into the nascent Woronin body (WB), driven by the self-assembly of Hex1. Recycling of Pex5 back to the cytosol occurs preferentially on the WB membrane, mediated by FAM1 (i.e., Pex22), Pex4, Pex26, Pex1, and Pex6.