FIG 5 .

Interaction of BAD-1 coated beads with RAW macrophages. (A) Binding of 5-µm-diameter polystyrene beads coated with BAD-1 or PVP (control) after 20 min of incubation with RAW macrophages. The y axis shows the number of beads bound per high-power field, which contained ≈300 macrophages. Assay results shown are representative of three separate experiments. Soluble heparin was added during coincubation with beads. Binding in the presence of 10 µg ml⁻¹ heparin was not significantly different from that for the negative control (PVP). (B) RAW macrophages were preincubated for 5 min with the PDI inhibitor DTNB. (C) RAW macrophages were preincubated for 5 min with the PDI inhibitors Rutin, DTNB, bisphenol, or PACMA31 before the addition of yeast. Strain 26199 is BAD-1 sufficient; strain 55 is BAD-1 deficient.