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(Top): Sensorgrams of solution heparin oligosaccharides/surface heparin competition. TIMP-3 concentration was 10 nM, and concentrations of heparin oligosaccharides in solution were 1000 nM. (Bottom): Bar graphs (based on triplicate experiments with standard deviation) of normalized TIMP-3 binding preference to surface heparin by competing with different sizes of heparin oligosaccharides in solution.
