Figure 1.

JQ1 treatment upregulates and activates SIRT1. JQ1 treatment for 24 h upregulates SIRT1 expression in A) mouse N9 microglia and B) A549, MCF-7 and HEK293 human cells. Cell lysates were analyzed by western blotting using SIRT1 and α-tubulin antibodies. C) JQ1 treatment enhances p53 deacetylation. A549, MCF-7 and HEK293 cells were treated for 24 h with JQ1 (400 nm), followed by a 5 h stimulation with etoposide (100 μm). Cell lysates were analyzed by western blotting with acetylated p53 and α-tubulin antibodies. Western blots are representative of at least three independent experiments.