Figure 2.

Effects of BET and SIRT1 inhibition on LPS-induced inflammatory response and ROS generation. A) JQ1 treatment diminished LPS-induced IL-8 secretion and reversed the effect of SIRT1 inhibition. A549 cells were treated with EX527 (5 μm), JQ1 (400 nm) and LPS (10 μg mL⁻¹) for 24 h. *: significant differences compared to treatment without LPS; #: significant differences between LPS-treated groups (p<0.05/Tukeys). B) Treatments with EX-527 or JQ1 had no significant effect on basal or LPS-stimulated ROS generation. A549 cells were treated with EX527 (5 μm), JQ1 (400 nm) and LPS (10 μg mL⁻¹) for 24 h. *: significant differences compared to treatment without LPS (p<0.05/Tukeys).