Table 2.
Expression patterns of the 12 mRNAs selected for qRT-PCR validation
| Gene name | qRT-PCR | RNA-Seq | ||
|---|---|---|---|---|
| Fold-changea (L30/L20b) | P-value | Fold-change (L30/L20) | P-value | |
| APAO1 | −0.48 | 0.008 | −0.34 | 0.004 |
| CEPT1 | 3.71 | 0.004 | 2.73 | 0.000 |
| CETP | −0.05 | 0.010 | −0.06 | 0.000 |
| PRDX | 0.61 | 0.056 | 0.94 | 0.732 |
| RPL6 | 0.71 | 0.281 | 1.31 | 0.177 |
| RPS24 | 0.97 | 0.192 | 1.27 | 0.177 |
| FOXO3 | 2.43 | 0.006 | 3.86 | 0.000 |
| SIRT1 | 2.62 | 0.054 | 1.32 | 0.087 |
| MTP | 1.51 | 0.068 | 1.12 | 0.537 |
| ApoB | 14.91 | 0.006 | 8.03 | 0.000 |
| LPGAT1 | −0.47 | 0.050 | −0.64 | 0.010 |
| ENSGALG- | 159.23 | 0.000 | 92.77 | 0.000 |
| 00000010018 | ||||
aMinus sign indicates the gene was down-regulated. bL30/L20, fold change in gene expression in liver samples from egg laying hens (L30) compared with liver samples from juveniles (L20)