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. 2015 Oct 9;15:669. doi: 10.1186/s12885-015-1676-0

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© Huang et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Examples of MS-HRM analysis of a APC, b MAL, c CDH13 and d RARβ genes in ipsilateral sample 1. The figure shows negative first derivative (T_m) melting curves of the MS-HRM profiles. MS-HRM differentiates the methylated DNA from the unmethylated DNA based on the sequence-dependent thermostability. Fully methylated samples melt later than the unmethylated WGA samples as there are cytosines retained in the sequence after bisulfite modification. Standards with different methylation levels (10 %, 25 % and 50 %) were prepared by mixing the fully methylated DNA with fully unmethylated DNA. Ipsilateral sample 1A represents the first tumour and 1B represents the second tumour