Table 2.

Strain-specific gene content based on the comparison of the draft genomes of C. diphtheriae strains ISS 3319, ISS 4060, ISS 4746 and ISS 4749

Protein annotation	ISS 3319	ISS 4060	ISS 4746	ISS 4749	ISS 4746 – ISS 4749a
Hypothetical	69	73	22	10	86
Fimbrial & membrane	4	4	-	2	12
Transposase	6	13	3	1	14
Othersb	24	31	5	2	37

^aStrains ISS 4746 and ISS 4749 belong to the same ST, ST32. Hence, the genes shared by them but not with ISS 3319 and ISS 4060 were treated as strain-specific and these are detailed in the column “ISS 4746 – ISS 4749”

^bCDS encoding transcription regulators, components of restriction-modification systems or other cellular and metabolic activities were grouped within the ‘Others’ category