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. 2015 Sep 7;3(9):e12496. doi: 10.14814/phy2.12496

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effects of 3-day subpressor AngII infusion on (A) renal cortical angiotensinogen expression, (B) urinary angiotensinogen excretion, and (C) natriuretic response to saline challenge. Immunoblots of cortical (A) and urinary (B) angiotensinogen of rats infused with either vehicle (Control o) or AngII (_▀) (n = 7). (A) 20 and 10 μg renal cortical protein loaded per lane. Relative abundance displayed as individual records with means ± SEM, (B) 0.02% of overnight urine volume before minipump implantation (day 1) and before sacrifice (day +3) displayed as paired arbitrary density units connected by either dotted lines (controls) or solid lines (AngII group); (C) at baseline, then repeated after 3 days of AngII infusion, rats were challenged with an i.p. bolus of saline equivalent of 7% of their body weight and placed in metabolic cages for urine collection (n = 4). Results are displayed as paired urinary Na⁺ excretion (UNaV) collected over 4 h, connected by solid lines.