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Short-term low-dose AngII infusion increases cortical but not medullary SPAK abundance and phosphorylation. Immunoblots of SPAK total (20 and 10 μg/lane) and SPAKpS373 (80 and 40 μg/lane in cortex, 20 and 10 μg/lane in medulla) in homogenates of renal cortex (A) and medulla (B) of rats infused with either vehicle (Control, ○) or AngII (▀) (n = 7). Relative abundance displayed as individual records with means ± SEM. *P < 0.05, #P = 0.05.
