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. 2015 Sep 14;3(9):e12547. doi: 10.14814/phy2.12547

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© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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SERCA1a (A) and SERCA2a (B) content were determined for middle aged control (CTL) and BSO-treated rat costal diaphragm using quantitative Western blotting analysis. Summary of data are shown in C (n = 12 per condition). Ca²⁺-dependent ATPase activity was measured in adult and middle aged diaphragm samples taken before and after (D) fatiguing stimulation. Note that Ca²⁺-ATPase data for adult BSO is not included here due to limited tissue availability. ATPase data were analyzed using a two-way mixed design ANOVA (between groups: adult control [n = 7] vs. middle aged control [n = 13] vs. middle aged BSO [n = 13]; repeated measures: basal vs. fatigued). Values are mean ± SEM. *Fatigued value < Basal value of the same group (P < 0.05). ^#Value > Basal Adult CTL (P < 0.05).