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. 2015 Sep 28;3(9):e12558. doi: 10.14814/phy2.12558

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Published 2015. This article is a U.S. Government work and is in the public domain in the USA. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Effect of TLR4 deficiency on splenocyte apoptosis, splenic PD-1 expression, and serum HMGB-1. (A) Spleen apoptosis measured by caspase-3 positive staining (N = 6–8/group). (B) Splenic PD-1 cell staining (N = 4/group). (C) Serum HMGB-1 levels (N = 7–8/group) in mice 4 weeks after 5/6Nx+AngII.