Table 2.
Real time RT-PCR reaction condition.
Initialization | Denaturation | Annealing | Elongation | Cycle | |
---|---|---|---|---|---|
DMP-1 | 95°C 10 min | 95°C 15 sec | 60°C 30 sec | 72°C 30 sec | 50 |
ALP | 95°C 10 min | 95°C 15 sec | 55°C 30 sec | 72°C 30 sec | 45 |
BSP | 95°C 10 min | 95°C 15 sec | 55°C 15 sec | 72°C 30 sec | 50 |
OCN | 94°C 10 min | 95°C 15 sec | 55°C 30 sec | 68°C 30 sec | 50 |
Runx-2 | 95°C 10 min | 95°C 15 sec | 55°C 30 sec | 72°C 40 sec | 45 |
β-actin | 95°C 10 min | 95°C 15 sec | 53°C 30 sec | 72°C 40 sec | 40 |