Table 1.
MRM parameters for the detection of DA, SRT and their deuterated internal standard compounds.
| Analyte | MRM transition [m/z] a | Collision energy [V] |
|---|---|---|
| Dopamine | 154.1 > 137.0* | 8 |
| 154.1 > 91.0 | 24 | |
| 154.1 > 65.0 | 40 | |
|
| ||
| Dopamine-d4 | 158.1 > 141.0* | 8 |
| 158.1 > 95.0 | 24 | |
| 158.1 > 68.0 | 40 | |
|
| ||
| Serotonin | 177.1 > 160.0* | 4 |
| 177.1 > 115.1 | 32 | |
|
| ||
| Serotonin-d4 | 181.1 > 164.1* | 4 |
| 181.1 > 118.1 | 32 | |
a
Values determined using the Optimizer tool of the Agilent MassHunter Workstation Software. Asterisks indicate mass transitions used for quantification. Additional mass transitions were recorded for structure identification of the analytes.