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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Talanta. 2015 May 28;144:71–79. doi: 10.1016/j.talanta.2015.05.057

Table 2.

Method validation parameters determined for the simultaneous quantification of DA and SRT in C. elegans homogenates.

Parametera Dopamine Serotonin
Recovery [%]b 87.4 ± 1.4 74.3 ± 0.8
Intraday variation [%]c 5.8 ± 1.4 6.3 ± 1.2
Interday variation [%]c 12.6 ± 3.5 11.5 ± 4.2
Limit of detection [nM]d 0.11 0.04
Limit of detection on column [fmol]d 0.56 0.20
Limit of quantification [nM]e 0.37 0.14
Limit of quantification on column [fmol]e 1.87 0.68
Linearity in range LOD – 2.5 pmol [r2]f 0.99984 0.99983
a

All method validation parameters were determined using matrix-matched samples (extract of 40,000 cat-2 or N2 worms per sample).

b

Data are means ± SE of 10 separate analyses.

c

|1 − (xi/x̄)| * 100 % with xi as result of each single measurement and x̄ as the mean result of all analyses. Data are means ± SE of 10 analyses (intraday variation) or 5 analyses (interday variation).

d

Defined as analyte amount that produces a peak with a S/N ratio of 3.

e

Defined as analyte amount that produces a peak with a S/N ratio of 10.

f

Worm extracts were spiked with fixed amounts of both deuterated internal standards as well as seven varying amounts of both analytes. The peak areas of the analytes, corrected for the responsiveness of the internal standards, were plotted against the concentrations of the analytes followed by linear regression analysis.