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. Author manuscript; available in PMC: 2016 Sep 22.
Published in final edited form as: Biochemistry. 2015 Sep 11;54(37):5669–5672. doi: 10.1021/acs.biochem.5b00911

Figure 1.

Figure 1

ChsE4-ChsE5 and MCR product analysis by MALDI-TOF mass spectrometry illustrating that ChsE4-ChsE5 is stereospecific for the 25S steroyl-CoA diastereomer. a) 1:1 (25R:25S)-3-OCS-CoA substrate (top), product of ChsE4-ChsE5 catalyzed dehydrogenation (middle), product after addition of MCR to the ChsE4-ChsE5 reaction mixture (bottom). b) 7:3 (25S:25R)-Δ7-dafachronyl-CoA substrate (top), product of ChsE4-ChsE5 catalyzed dehydrogenation (middle), product after addition of MCR to the ChsE4-ChsE5 reaction mixture (bottom). In each case, reaction mixtures were monitored until no further changes in product distribution occurred.