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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Glia. 2015 Jul 3;63(12):2168–2180. doi: 10.1002/glia.22883

FIGURE 5.

FIGURE 5

RNA polymerase III activity is required for maximal glial responses to a DAI ligand but not a RIG-I agonist. Mixed glial cells, EOC13.31 microglia-like cells, or primary murine astrocytes were untreated (open bars) or pretreated with a RNA polymerase III inhibitor (25 uM) for 24 hrs (black bars) prior to being untransfected (0), treated with transfection reagent alone (CON), or transfected with uncapped 5′ppp-dsRNA (4 ug/ml) or B-DNA (3 ug/ml). At 24 hrs following transfection, expression of mRNA encoding IFN-β was determined by qPCR. Data are expressed as the mean +/− SEM of a representative experiment, performed in triplicate, of studies that were performed three independent times. An asterisk indicates a statistically significant effect of RNA polymerase III inhibition on IFN-β expression.