Skip to main content
. Author manuscript; available in PMC: 2016 Sep 7.
Published in final edited form as: Cytoskeleton (Hoboken). 2015 Sep 7;72(8):422–433. doi: 10.1002/cm.21237

Fig. 7.

Fig. 7

Effect of zIQGAP3 KD on convergent extension movements. (A–D) In situ hybridization at the 3-somite stage with the combination of hgg1 (hatching gland gene 1, a marker for anterior prechordal plate – app), dlx3 (distal-less homeobox3, a marker of neural plate border – npb) and ntl (notail, a marker of the notochord – n) in a WT embryo (A), and embryos injected with Wnt11 MO (n = 105) (B) or zIQGAP3 MO (n = 121) (C,D). Compared to WT, Wnt11 and IQGAP3 morphants display a wider neural plate and notochord, and an anterior prechordal plate posterior to the neural plate border. These different defects signify disruption of the convergent extension movements occurring at gastrula stage. (E–F) In situ hybridization with myod1 (myogenic differentiation 1), which labels the adaxial cells (ad) as well as the border of the somites in (E) WT and (F) IQGAP3 morphant embryos at the 12-somite stage (n = 108). Embryos are presented dorsal view (head region), anterior to the left.