Table 2.
Pms1-foci abundance in different MMR-related mutants in S. cerevisiae
| Biochemical defect | Relevant genotype | Mutator phenotype (frameshift-reversion assay) | % Pms1 focia | Reference |
|---|---|---|---|---|
| None (wild-type strain) | Wild-type | None | 10 | Hombauer et al. 2011a, b |
| 1. Reduced DNA replication fidelity | ||||
| (a) exonuclease defective polymerases | pol3-01; pol2-04 | None | 20–25 | Hombauer et al. 2011a |
| (b) polymerase active-site mutation | pol3-L612M | None | 20 | Hombauer et al. 2011a |
| 2. Mutations that interfere with MMR | ||||
| (a) Preventing mispair recognition | msh2Δ; msh3Δmsh6Δ | Strong | 0 | Hombauer et al. 2011a |
| (b) Msh6 dominant mutations (in an msh3Δ background) | S1036P; G1067D; G1142D | Strong | 0 | Hombauer et al. 2011a |
| (c) Excision (5′-3′ Exo1-dependent) | exo1Δ | Weak | 50 | Hombauer et al. 2011a |
| (d) Nicking reaction | ||||
| PCNA (Pol30) mutations that prevent Pms1 endonuclease activation | K13E; C22Y; K217E | Moderate | 60–95 | Goellner et al. 2014 |
| Pms1 endonuclease defective mutations | G683E, E707K, C817R, C848S | Strong | 60–85 | Smith et al. 2013 |
| Mlh1 endonuclease defective mutation | E767stop | Strong | 95 | Smith et al. 2013 |
a
Percentage of yeast cells containing Pms1-foci on a logarithmically growing population for the indicated genotypes