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. 2015 Oct 1;6:8399. doi: 10.1038/ncomms9399

Figure 4. Cohesin levels determine sensitivity to APC/C inhibition.

Figure 4

(a–d) DDX11− cells were first transfected with either non-targeting or WAPL siRNA, to obtain an efficient knockdown. Two days later, cells were again transfected with the indicated siRNAs. At day 3 after the second transfection round, cells were harvested for western blot (a), cohesion defect analysis (b), and flow cytometry (c). At day 4, viability was measured with a CellTiter-Blue assay (d). Viability upon siAPC3 only was significantly different from siWAPL+siAPC3 (P=0.002 using a students t-test), indicated by an asterisk. (e–h) RPE1 cells were transfected with the indicated siRNAs. Western blot (e) cohesion defect analysis (f) and flow cytometry (g) were performed 2 days after transfection. Means of two independent experiments are shown. The asterisk indicates non-specific background staining. At day 4, viability was measured with a CellTiter-Blue assay (h). A students t-test revealed P=0.057 when comparing viability upon siAPC3 only and siESCO2+siAPC3. Error bars denote standard deviations of three technical replicates.