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. 2015 Oct 1;6:8434. doi: 10.1038/ncomms9434

Figure 8. ADMP1 and ADMP2 are required for high level of BMP signalling and cooperate with BMP2/4 to build the dorsal apex.

Figure 8

(a) Morphological phenotypes caused by knocking-down ADMP1 or ADMP2 with morpholino oligonucleotides. Injection of morpholinos targeting the translation start site (Mo1) or the 5' UTR (Mo2) of admp1 or admp2 disrupts D/V patterning resulting in embryos that do not elongate along the ventral or dorsal sides. (b) Knocking-down admp1 with admp1-Mo2 increases the size of the chordin expressing territory consistent with the idea that ADMP1 antagonizes Nodal. The dorsal marker gene msx is also downregulated in admp1 morphants. (c) Knocking-down admp2 with admp2-Mo1 specifically eliminated the expression of marker genes expressed in the dorsal-vegetal and lateral ectoderm including fgfA, pax2/5/8, irxA and msx and suppressed expression of sm30 in the PMC clusters. (d)Morpholinos targeting either admp1 or admp2 strongly reduced pSmad1/5/8 staining in the presumptive dorsal-vegetal ectoderm and mesoderm but knocking-down admp2 with admp2-Mo1 more strongly diminished pSmad1/5/8 staining in the PMCs compared with knocking-down admp1 with admp1-Mo2. Scale bar, 10 μm.