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. 2015 Oct 1;6:8434. doi: 10.1038/ncomms9434

Figure 9. ADMP1 and ADMP2 act in synergy with BMP2/4 to repress formation of the ciliary band on the dorsal side.

Figure 9

(a) Blocking translation of BMP2/4 causes a dramatic expansion of the ciliary band on the dorsal side while partially inhibiting BMP2/4 causes a truncation of the dorsal apex. Co-injection of low doses of the bmp2/4 together with low, sub-optimal, doses of the admp1 or admp2 morpholinos produces a strong BMP loss-of-function phenotype indicating that these two ADMP ligands cooperate with BMP2/4 during D/V axis formation. Note the similarity of the phenotypes of embryos injected with high doses of the BMP2/4 morpholino or co-injected with the admp1+bmp2/4 or admp2+bmp2/4 morpholinos at sub-optimal doses. (b) Expression of chordin, onecut and hox7 in embryos co-injected with low doses of the bmp2/4 and admp1 or bmp2/4 and admp2 morpholinos. Note the dramatic dorsal expansion of the ciliary band marker onecut, the loss of hox7 and the partial expansion of chordin following co-injection of either combination of the two morpholinos at low doses. Scale bar, 10 μm.