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. 2015 Oct 5;6:8524. doi: 10.1038/ncomms9524

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HeLa cells were starved in EBSS for 2 h and then stimulated with essential amino acids (EAAs) for indicated time intervals in the presence of (a) UPS_6.2/UPS_5.3/UPS_5.0 and (b) UPS_4.7/UPS_4.4. Water and 50 μM chloroquine (CQ) were used as control. Accumulation of the indicated phosphoproteins was assessed by immunoblot of whole-cell lysates. (c) Quantitative analysis of the nuclear/cytosolic distribution of GFP-TFEB following the indicated treatments. Error bars represent s.d., n=10. (d) Representative images for c. Scale bar, 10 μm. (e) Working model of pH transitions required for free amino acid versus albumin-derived amino-acid-dependent activation of the mTORC1 signalling pathway.