Figure 2. Conditional inhibition of dermal Shh expression led to reduced dentate progenitors.
(A) Krt14-Cre was used to conditionally delete Shh expression (Krt14-cre;Shhflx/flx, in short Mut in this figure). Expression of Ptch1, a downstream target gene of Shh signaling, and Shh showed absence of Shh expression in the mutant HFs. The right panel shows Ptch1 expression in the forebrain at E17.5. (B) Expression of a dentate progenitor marker Ki67 at E17.5 and P1. Pdgfrα and Reelin show meningeal cells and Cajal Retzius cells outlining the dentate gyrus (DG), respectively. Prox1 shows dentate granule neurons at E18.5. (B′, B″) Plots show Ki67 + cells in the dentate at E17.5 (B′) and P1 (B″). Student t-test was used to determine the significant difference between groups. Scale bars: A = 200 μm, B = 100 μm. p = 0.03 (B′), <0.05 (B″). Dashed lines were used to outline the dentate.
Figure 2—figure supplement 1. A representative image for in situ hybridization of Ptch1 using P1 Krt14-Cre;Shhflx mice.
