Fig. 7.

Clustering of cell line transcription factor profile and correlation of transcription factor to observed phenotype. (A) Average transcription factor expression matrix and clustering of treated cell lines based on 48 h nuclear transcription factor expression data. Mean quantified Western blot data for each cell line treatment case was subject to hierarchical clustering analysis using the Pearson correlation coefficient as a distance metric and using an average linking method. (B) Correlation and clustering without VDR, IRF-1 or RARα. (C–E) Pearson correlation coefficient matrix between average transcription factor expression and phenotypic results at 48 h across all cell lines for (C) RA treatments, (D) D3 treatments and (E) RA + D3 treatments. Hierarchical clustering of phenotypic results using average linkage for all cell lines across (F) RA treatments, (G) D3 treatments and (H) RA + D3 treatments.