Table 1. The DNA Amplification Program and the Specific Primers Used for Standard PCR Procedure.

Target	Primer Sequence	Amplicon Size, bp	Initial Denaturation	Second Denaturation	Annealing Stage, for 1 min	First Extension	Last Extension
GES			94ºC for 4 min	35 cycles of denaturation at 94ºC for 1 min	60ºC	72ºC for 1 min	72ºC for 10 min
	F (5’-ATGCGCTTCATTCACGCAC-3’)	864
	R (5’-CTATTTGTCCGTGCTCAGG -3’)
PER			94ºC for 4 min	35 cycles of denaturation at 94ºC for 1 min	55ºC	72ºC for 1 min	72ºC for 10 min
	F (5’-ATG AAT GTC ATT ATA AAA GC -3’)	925
	R (5’-AAT TTG GGC TTA GGG CAG AA-3’)
SHV			94ºC for 4 min	35 cycles of denaturation at 94ºC for 1 min	58ºC	72ºC for 1 min	72ºC for 10 min
	F(5’-AAGATCCACTATCGCCAGCAG-3’)	231
	R (5’-ATTCAGTTCCGTTTCCCAGCGG-3’)
TEM			94ºC for 4 min	35 cycles of denaturation at 94ºC for 1 min	55ºC	72ºC for 1 min	72ºC for 10 min
	F (5’-ATGAGT ATTCAACATTTCCG-3’)	858
	R (5’-CCAATGCTTAATCAGTGAGG-3’)
OXA-10			94ºC for 4 min		58ºC	72ºC for 1 min	72ºC for 10 min
	F (5’-TCAACAAATCGCCAGAGAAG-3’)	276
	R (5’-TCCCACACCAGAAAAACCAG-3’)
KPC			94ºC for 4 min	35 cycles of denaturation at 94ºC for 1 min	52ºC	72ºC for 1 min	72ºC for 10 min
	F (5’-ATGTCACTGTATCGCCGTCT-3’)	893
	R (5’-TTTTCAGAGCCTTACTGCCC-3’)