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NMR data recorded on NS5A-FL. (a) 1H-15N BEST-TROSY spectrum recorded at 5°C and 950 MHz. (b) Overlay of 13C′ and 13Cα secondary chemical shifts for NS5A-D2D3 (black) and NS5A-FL (blue). Peptide regions showing missing or low cross-peak intensities in NS5A-FL are highlighted (gray bars). To see this figure in color, go online.
