Figure 2.

Identification of positions in TetL correlated with increased native growth temperature. (A) Topology of BsTetL. Positions tested are boxed in blue and magenta for global and pairwise predictions, respectively. (B) Heatmap of positional amino acid conservation by the global method corresponding to helices 1–6; data for the entire protein are shown in Fig. S2. Positive values indicate overrepresented in thermophiles, and negative values indicate underrepresentation (or overrepresented in mesophiles). (C) Frequency of amino acid change as a function of relative OGT differences for helices 1–6; data for the entire protein are shown in Fig. S4. Positions of low conservation (>50% gaps) are hidden. To see this figure in color, go online.