Figure 3.
Overexpression and stability of single BsTetL mutants. (A) Fluorescence of wild-type BsTetL-GFP and single mutants. Mutants predicted by the global and pairwise methods are colored blue and magenta, respectively. The horizontal bar indicates wild-type BsTetL fluorescence. (B) Residual fluorescence (fractional fluorescence that remains after 42°C thermal stress) of BsTetL-GFP and single mutants. Mutants predicted by the global and pairwise methods are colored blue and magenta, respectively. The stability of wild-type BsTetL is indicated by the horizontal bar. To see this figure in color, go online.