Overexpression and stability of select double and triple BsTetL mutants. (A) Fluorescence of BsTetL-GFP and mutants. Single, double, and triple mutants are colored orange, red, and maroon, respectively. The horizontal bar indicates wild-type BsTetL-GFP fluorescence. (B) Residual fluorescence of BsTetL-GFP and mutants after 42°C thermal stress. Single, double, and triple mutants are colored orange, red, and maroon, respectively. The stability of wild-type BsTetL is indicated by the horizontal bar. To see this figure in color, go online.