In vitro stability and tetracycline resistance of BsTetL mutants. (A) Thermal denaturation of BsTetL and mutants. (B) Growth curve of BsTetL and mutants in the presence of tetracycline at 37°C. Single, double, and triple mutants are colored orange, red, and maroon, respectively. Wild-type and empty vector control are indicated by open squares and circles, respectively. To see this figure in color, go online.