Table 1.

Potential TetL-stabilizing mutations identified by the global method

Position	Thermophilic AA	fThermo(AA,i)	fMeso(AA,i)	p-Value	TetL Mutation	Successful
31	S	0.393	0.149	2.44E-10	M31S
94	A	0.557	0.318	1.01E-23	I94A
124	A	0.443	0.207	1.18E-14	V124A
126	A	0.614	0.308	5.28E-23	V126A	+
132	F	0.714	0.415	5.38E-33	I132F
134	P	0.550	0.307	9.80E-23	K134P
167	G	0.514	0.279	2.90E-20	H167G
173	Y	0.414	0.173	4.36E-12	L173Y
215	L	0.614	0.333	4.26E-25	M215L	+
394	N	0.243	0.117	4.46E-08	F394N	+

Overrepresented amino acids by position in the thermophilic sequences are noted with the frequency of that amino acid in both the thermophilic and mesophilic sequences at that same position, with corresponding p-values calculated by the binomial test. A mutant was considered successful if the residual fluorescence, after thermal stress, was significantly greater than that of the wild-type.