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. 2015 Oct 6;109(7):1420–1428. doi: 10.1016/j.bpj.2015.07.026

Table 2.

Potential TetL-stabilizing mutations identified by the pairwise method

Position fdiff(i: |ΔOGT| ≥ Tthreshold ∧ I ≥ Ithreshold) fdiff(i: I > Ithreshold) TetL Mutation Successful
109 0.652 0.279 A109F
131 0.611 0.244 Y131V
184 0.656 0.290 F184L +
208 0.714 0.259 S208T
232 0.795 0.448 A232F
233 0.712 0.363 F233L
241 0.832 0.338 R241K
250 0.569 0.183 P250L +
270 0.614 0.248 G270V
335 0.819 0.435 S335L +
341 0.692 0.332 A341S +
350 0.699 0.342 I350T
353 0.815 0.461 I353L
358 0.641 0.219 V358L
436 0.788 0.364 F436I

The frequency of a difference at a position is listed for both the presence and absence of a significant OGT difference between pairs. A mutant was considered successful if the residual fluorescence, after thermal stress, was significantly greater than that of the wild-type.