Table 2. Curve Parameters.
| Substance | A1(tone) | A2(contraction) | DA2 – A1 | |
|---|---|---|---|---|
| DMSO | 98.96 ± 2.68 | 89.65 ± 2.68 | −9.13 ± 2.68 | |
| isoprotenerol | 102.34 ± 2.75 | 91.14 ± 2.75 | −11.12 ± 2.75 | |
| tyramine | 98.08 ± 2.94 | 77.82 ± 2.94 | −20.03 ± 2.94 | |
| salbutamol | 95.98 ± 3.02 | 81.29 ± 3.02 | −14.48 ± 3.02 | |
| epinastine | 97.31 ± 1.73 | 84.74 ± 1.73 | −12.39 ± 1.73 | |
| clonidine | 99.73 ± 3.75 | 86.35 ± 3.75 | −13.38 ± 3.75 | |
| prazosin | 95.75 ± 3.38 | 83.64 ± 3.38 | −11.97 ± 3.38 | |
| amitraz | 98.33 ± 5.05 | 87.31 ± 5.05 | −11.02 ± 5.05 | |
| octopamine | 102.06 ± 4.81 | 86.62 ± 4.81 | −15.43 ± 4.81 | |
| Statistical significance of Tone (A1), Contraction (A2) and Whole Curves | ||||
| ANOVA Dunnett's multiple comparisons test | Mean Diff. | 95% CI of diff. | Significant? | Summary |
| Tone (A1) | ||||
| DMSO vs. Isoprotenerol | −3.55 | −3.77 to −3.32 | Yes | **** |
| DMSO vs. Tyramine | 1.15 | 0.925 to 1.37 | Yes | **** |
| DMSO vs. Salbutamol | 3.22 | 3.00 to 3.44 | Yes | **** |
| DMSO vs. Epinastine | 1.83 | 1.61 to 2.06 | Yes | **** |
| DMSO vs. Clonidine | −0.806 | −1.03 to −0.584 | Yes | **** |
| DMSO vs. Prazosin | 3.32 | 3.10 to 3.54 | Yes | **** |
| DMSO vs. Amitraz | 0.624 | 0.402 to 0.846 | Yes | **** |
| DMSO vs. Octopamine 5 μM | −0.522 | −0.744 to −0.300 | Yes | **** |
| DMSO vs. Octopamine 1.7 μM | 0.102 | −0.120 to 0.324 | No | Ns |
| DMSO vs. Octopamine15 μM | −3.21 | −3.43 to −2.99 | Yes | **** |
| Contraction (A2) | ||||
| DMSO vs. Isoprotenerol | −1.99 | −2.38 to −1.61 | Yes | **** |
| DMSO vs. Tyramine | 11.2 | 10.8 to 11.5 | Yes | **** |
| DMSO vs. Salbutamol | 7.75 | 7.37 to 8.14 | Yes | **** |
| DMSO vs. Epinastine | 4.42 | 4.03 to 4.80 | Yes | **** |
| DMSO vs. Clonidine | 2.55 | 2.17 to 2.94 | Yes | **** |
| DMSO vs. Prazosin | 5.85 | 5.47 to 6.24 | Yes | **** |
| DMSO vs. Amitraz | 2.45 | 2.07 to 2.84 | Yes | **** |
| DMSO vs. Octopamine 5 μM | 5.17 | 4.79 to 5.56 | Yes | **** |
| DMSO vs. Octopamine 1.7 μM | 1.7 | 1.31 to 2.08 | Yes | **** |
| DMSO vs. Octopamine15 μM | 6.09 | 5.70 to 6.47 | Yes | **** |
| Whole Curve (tone and contraction phases) | ||||
| DMSO vs. Isoprotenerol | −2.77 | −3.30 to −2.24 | Yes | **** |
| DMSO vs. Tyramine | 6.16 | 5.63 to 6.69 | Yes | **** |
| DMSO vs. Salbutamol | 5.49 | 4.96 to 6.02 | Yes | **** |
| DMSO vs. Epinastine | 3.13 | 2.60 to 3.66 | Yes | **** |
| DMSO vs. Clonidine | 0.874 | 0.345 to 1.40 | Yes | **** |
| DMSO vs. Prazosin | 4.59 | 4.06 to 5.12 | Yes | **** |
| DMSO vs. Amitraz | 1.54 | 1.01 to 2.07 | Yes | **** |
| DMSO vs. Octopamine 5 μM | 2.33 | 1.80 to 2.86 | Yes | **** |
| DMSO vs. Octopamine 1.7 μM | 0.901 | 0.371 to 1.43 | Yes | **** |
| DMSO vs. Octopamine15 μM | 1.44 | 0.913 to 1.97 | Yes | **** |
In all cases: n = 6 *P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001, alpha 0.05.