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. 2015 Oct 12;5:15049. doi: 10.1038/srep15049

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Copyright © 2015, Macmillan Publishers Limited

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PMA differentiated THP-1 cells were co-cultured separately with live and heat killed H. pylori P12 and 26695 strains for 24 h and Mincle mRNA expression was quantified by qRT-PCR.H. pylori strains were heat killed at 98 °C for 20 min. Mincle mRNA expression was normalized with PPIB (peptidlypropylisomerase) mRNA expression. Mincle expression presented as relative to un-infected THP-1 cells. Data are mean ± s.e.m of three independent experiments, and were analysed by one way ANOVA followed by Tukey’s multiple comparison test.