Figure 3.

(a) Schematic representation of experimental design: PMA differentiated THP-1 cells were cultured at the bottom of the trans-well plate and H. pylori were separated from cells on the upper compartment having 0.4micron filter (In-direct infection).Uninfected control cells and cells infected directly with H. pylori (direct infection) were cultured normally in a 6 well plate. (b) Mincle expression is independent of THP-1 cells contact: Differentiated THP-1 cells were infected with H. pylori for 24 h and Mincle transcription was quantified by qRT-PCR. No significant fold change was observed in Mincle transcription level between directly infected THP-1 cells and indirectly infected THP-1 cells (separated). Results are presented relative to directly infected THP-1 cells and were normalized with PPIB housekeeping gene. Data are representative of three independent experiments (mean ± s.e.m) and analysed by student’s t test. (c) Quantitative RT-PCR analysis of TNF-a and IL-10 in THP-1 cells: TNF-a and IL-10 mRNA levels were quantified in both directly infected and indirectly infected THP-1 cells (separated) after 24 h. Mincle mRNA levels were normalized by PPIB as endogenous control and shown as fold change relative to directly infected THP-1 cells.